The manuscript describes validated RP-HPLC method for simultaneous estimation of domperidone, tramadol hydrochloride and dicyclomine in tablet dosage form. The separation was achieved by C18 column (250×4.6 mm, 5 μm Particle Size) with mobile phase of 0.02 M KH2PO4, pH 6.5 Adjusted with NaOH (65:35%v/v) at 215 nm. Retention time of domperidone, tramadol hydrochloride and dicylomine was found to be 2.187 min, 2.827 min and 9.200 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity was found in the range of 5-30 μg/ml, 25-150 μg/ml and 5-30 μg/ml for domperidone, tramadol hydrochloride and dicylomine respectively. The recoveries for domperidone, tramadol hydrochloride and dicylomine were found to be of 100.01±0.04%, 101.05±0.33% and 99.00±0.02% respectively in tablet. Developed method was found to be new, accurate, precise and rapid for simultaneous estimation of domperidone, tramadol hydrochloride and dicylomine in tablet dosage form.
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